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These are our most up-to-date DNA Isolation Protocols for generating ultra-high molecular weight DNA.
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Generation 2 Tech Note for Fresh and Cryopreserved Amnio/CVS isolation of DNA.

The Bionano Prep SP-G2 Fresh and Cryopreserved Amnio and CVS DNA Isolation Tech Note is not a fully validated protocol. This tech note was evaluated using patient-derived cultured amniocyte and CVS cell cultures. gDNA prepared using this protocol has been validated only with Direct Label and Stain Generation 2 (DLS-G2) labeling.

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The Bionano Prep Methanol Glacial Acetic Acid Cell Preparation Tech Note is not a fully validated protocol. The technique has only been used to isolate UHMW gDNA from the Epstein-Barr virus (EBV) immortalized human lymphoblastoid cell line (GM12878) that has undergone a hypotonic swell and fixation, as described here, for the Bionano Saphyr system.

support documentation

This Bionano Prep SP Cryopreserved Cell Tech Note is not a fully validated protocol and has only been used to isolate ultra-high molecular weight (UHMW) DNA from cryopreserved lymphoblastoid cell lines, peripheral blood mononuclear cells (PBMC), and bone marrow mononuclear cells (BMNC) for the Bionano Saphyr system.

support documentation

This tech note is not a fully validated protocol. It has only been used to isolate UHMW DNA from non-tumorous healthy adult rat brain tissue and non-tumorous human brain tissue for the Bionano Saphyr system.

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