| OBSERVATION(S) | POTENTIAL CAUSE | OPTIONS TO RESOLVE |
|---|---|---|
| Low DNA recovery from membrane as determined by post-stain Qubit HS quantitation | DNA is too viscous to fully remove from membrane using the recommended pipette setting in the protocol | Set the pipette to 50 μl (or up to 100 μl) and move the tip back and forth across the membrane to ensure all DNA is removed |
| DNA is non-homogenous before beginning DLS | Perform additional mixing of the DNA with a wide-bore pipette tip (5x up and down). Allow DNA to homogenize at room temperature overnight. Repeat quantitation with the Qubit BR assay and then repeat DLS if the concentration is within 36 – 150 ng/μl with CV < 0.3 |
| OBSERVATION(S) | POTENTIAL CAUSE | OPTIONS TO RESOLVE |
|---|---|---|
| Average N50 ≥ 150kbp is < 230 kbp | Nuclease contamination | Ensure that only nuclease-free water is used for all protocol steps. Note: water provided in Bionano Kits is nuclease-free. |
| Freeze-thaw cycles | Take care to avoid any additional freeze-thaw cycles of starting sample. |
The intention of this guide is to provide suggestions for resolution of issues that can affect optimum performance regarding a specific sample type or workflow. These suggestions have not been validated by Bionano and instead come from customer feedback and suggested best-practice. Some recommended options include the use of additional reagent volume, which may reduce the total number of reactions per kit.
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This guide provides suggestions for resolution of errors that can affect optimum performance regarding a specific sample type or workflow. These suggestions have not been validated by Bionano. Some recommended options include the use of additional reagent volume, which may reduce the total number of reactions per kit.
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This document describes the major differences between the plant DNA isolation protocols and assists users in selecting the proper DNA isolation protocol for their plant sample.
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Find a variety of support documents for our products, including protocols, tech notes, guides, release notes, etc.
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