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This Bionano Prep SP-G2 Blood & Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours from human blood or cultured cells. It utilizes an improved lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA

kit contents

PART NO. Component Volume Storage SDS
20442 RBC Lysis 18 ml 15-30°C Download
20374 Cell Buffer 50 ml 15-30°C Download
20443 Digestion Enhancer 4.0 ml 15-30°C Download
20444 Lysis and Binding Buffer (LBB) 1.2 ml 15-30°C Download
20445 Wash Buffer 1 (WB1) 4.5 ml 15-30°C Download
20446 Wash Buffer 2 (WB2) 6.0 ml 15-30°C Download
20378 Elution Buffer (EB) 1.1 ml 15-30°C Download
20447 DE Detergent 55 μl 15-30°C Download
20448 4mm Nanobind Disks 12 ea. 15-30°C
20449 Protein LoBind Microcentrifuge Tubes, 1.5 ml 2 x 12 ea. 15-30°C
20450 Protein LoBind Microcentrifuge Tubes, 0.5 ml 12 ea. 15-30°C
20451 Magnetic Retriever Plastic Sheath 12 ea. 15-30°C
20452 Microcentrifuge Tubes, 2 ml 12 ea. 15-30°C
20423 DNA Stabilizer 350 μl 15-30°C Download
20455 RNase A 150 μl 2°C-8°C Download
20355 Ultra Pure Water 2 x 900 μl 2°C-8°C Download
20441 Thermolabile Proteinase K (TLPK) 150 μl -15°C to -25°C Download

related support materials

Bionano Prep SP-G2 Frozen Human Blood DNA Isolation Protocol - CG-00006, Rev B

Bionano Prep SP-G2 Fresh Human Blood DNA Isolation Protocol - CG-00005​, Rev C

Bionano Prep SP-G2 Frozen Cell Pellet DNA Isolation Protocol - CG-00004​, Rev B

Bionano Prep SP-G2 Fresh Cell Pellet DNA Isolation Protocol - CG-00003, Rev B

Cell Line Shipping Instructions - 30180, Rev C

Whole Blood Collection, Storage, and Shipping Instructions - 30179, Rev D

HemoCue WBC Analyzer Demo

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