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This Bionano Prep SP-G2 Blood & Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours from human blood or cultured cells. It utilizes an improved lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA

kit contents

PART NO. Component Volume Storage SDS
20442 RBC Lysis 2 x 18 ml 15-30°C Download
20374 Cell Buffer 50 ml 15-30°C Download
20457 Digestion Enhancer 6.5 ml 15-30°C Download
20458 Lysis and Binding Buffer (LBB) 2.0 ml 15-30°C Download
20459 Wash Buffer 1 (WB1) 13.0 ml 15-30°C Download
20460 Wash Buffer 2 (WB2) 17.5 ml 15-30°C Download
20463 Elution Buffer (EB) 3.6 ml 15-30°C Download
20545 DE Detergent 110 μl 15-30°C Download
20539 4mm Nanobind Disks 24 ea. 15-30°C
20540 Protein LoBind Microcentrifuge Tubes, 1.5 ml 2 x 24 ea. 15-30°C
20541 Protein LoBind Microcentrifuge Tubes, 0.5 ml 24 ea. 15-30°C
20543 Magnetic Retriever Plastic Sheath 24 ea. 15-30°C
20542 Microcentrifuge Tubes, 2 ml 24 ea. 15-30°C
20469 DNA Stabilizer 700 μl 15-30°C Download
20462 RNase A 300 μl 2°C-8°C Download
20532 Ultra Pure Water 2 x 1800 μl 2°C-8°C Download
20456 Thermolabile Proteinase K (TLPK) 325 μl -15°C to -25°C Download

related support materials

Bionano Prep SP-G2 Frozen Human Blood DNA Isolation Protocol - CG-00006, Rev D

Generation 2 SP DNA Isolation Protocol. To be used only with PN 80060 – Bionano Prep SP-G2 Blood and Cell DNA Isolation Kit.

Also available in FrenchGermanItalian, and Spanish.

Bionano Prep SP-G2 Fresh Human Blood DNA Isolation Protocol - CG-00005​, Rev E

Generation 2 SP DNA Isolation Protocol. To be used only with PN 80060 – Bionano Prep SP-G2 Blood and Cell DNA Isolation Kit.

Also available in FrenchGermanItalian, and Spanish.

Bionano Prep SP-G2 Frozen Cell Pellet DNA Isolation Protocol - CG-00004​, Rev D

Generation 2 SP DNA Isolation Protocol. To be used only with PN 80118 or 80060 – Bionano Prep SP-G2 Blood and Cell DNA Isolation Kit.

Also available in FrenchGermanItalian, and Spanish.

Bionano Prep SP-G2 Fresh Cell Culture DNA Isolation Protocol - CG-00003, Rev D

Generation 2 SP DNA Isolation Protocol. To be used only with PN 80118 or 80060 – Bionano Prep SP-G2 Blood and Cell DNA Isolation Kit.

Also available in FrenchGermanItalian, and Spanish.

Cell Line Shipping Instructions - 30180, Rev C

Whole Blood Collection, Storage, and Shipping Instructions - 30179, Rev D

HemoCue WBC Analyzer Demo

To view this video in another language, please click on one of the available languages below:
French | German
Italian | Spanish

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