SP Blood and Cell Culture DNA Isolation Kit

Part Number 80042

This Bionano Prep SP Blood and Cell Culture DNA Isolation Kit can provide ultra-high molecular weight (UHMW) gDNA in less than 4 hours for EDTA-collected blood and mammalian cell cultures. It utilizes a lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

Kit Contents

Part No.	Component	Volume	Storage	SDS
20373	RNase A Enzyme	200 μl	4°C	Download
20423 / 20397	DNA Stabilizer	350 μl	18-25°C	Download
20374	Cell Buffer	50 ml	18-25°C	Download
20372	Proteinase K Enzyme	0.5 ml	18-25°C	Download
20375	Lysis and Binding Buffer (LBB)	2.5 ml	18-25°C	Download
20376	Wash Buffer 1 Concentrate (2.5X) (WB1)	3.25 ml	18-25°C	Download
20377	Wash Buffer 2 Concentrate (2.5X) (WB2)	5 ml	18-25°C	Download
20378	Elution Buffer (EB)	1.1 ml	18-25°C	Download
20402	4 mm Nanobind Disks	10 disks	18-25°C
20422	Protein LoBind Tubes, 1.5 mL	10 tubes	18-25°C
20421	Protein LoBind Tubes, 0.5 mL	10 tubes	18-25°C
20396	Standard Microfuge Tubes, 2.0 ml	10 tubes	18-25°C
20381	Magnetic Disk Retriever Plastic Sheath	10 each	18-25°C

related support materials

	Bionano Prep SP Frozen Cell Pellet DNA Isolation Protocol v2 - 30398, Rev B To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2. Also available in French, German, Italian, and Spanish
	Bionano Prep SP Fresh Cells DNA Isolation Protocol v2 - 30396, Rev B To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2. Also available in French, German, Italian, and Spanish
	Bionano Prep SP Frozen Human Blood DNA Isolation Protocol v2 - 30395, Rev B To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2. Also available in French, German, Italian, and Spanish
	Bionano Prep SP Fresh Human Blood DNA Isolation Protocol v2 - 30397, Rev B To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2. Also available in French, German, Italian, and Spanish
	Bionano Prep SP Amnio and CVS Culture DNA Isolation Protocol - 30403, Rev A To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2.
	Bionano Prep SP Cryopreserved Cell Tech Note - TECHN-00001, Rev 01 To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2.
	Bionano Prep Methanol Glacial Acetic Acid Fixed Cell Preparation Technical Note - TECHN-00008, Rev A To be used only with PN 80042 – SP Blood & Cell Culture DNA Isolation Kit v2.
	Cell Line Shipping Instructions - 30180, Rev C
	Whole Blood Collection, Storage, and Shipping Instructions - 30179, Rev D
	HemoCue WBC Analyzer Demo To view this video in another language, please click on one of the available languages below: French \| Spanish \| Italian \| German
	Bionano Prep SP Frozen Blood DNA Isolation Demo To view this video in another language, please click on one of the available languages below: French \| Spanish \| Italian \| German

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