| OBSERVATION | POTENTIAL CAUSE | OPTION TO RESOLVE |
|---|---|---|
| Low labeled DNA recovery from DLS membrane, as determined by Qubit HS assay | gDNA is non-homogenous before beginning DLS-G2 protocol | Perform additional mixing of the gDNA with a wide-bore pipette tip. Allow DNA to homogenize at room temperature overnight. Repeat quantitation with the Qubit BR assay, ensure gDNA concentration is within 39 – 150 ng/μl with CV < 0.3, and repeat DLS-G2 protocol. |
| OBSERVATION(S) | POTENTIAL CAUSE | OPTIONS TO RESOLVE |
|---|---|---|
| Average N50 ≥ 150kbp is < 230 kbp | Freeze-thaw cycles | Take care to avoid any additional freeze-thaw cycles of starting sample. |
The intention of this guide is to provide suggestions for resolution of issues that can affect optimum performance regarding a specific sample type or workflow. These suggestions have not been validated by Bionano and instead come from customer feedback and suggested best-practice. Some recommended options include the use of additional reagent volume, which may reduce the total number of reactions per kit.
Download SP-G2 and DLS-G2 Troubleshooting Guide
PLEASE NOTE: This troubleshooting guide is for the previous generation protocols only! For troubleshooting related to the newest SP-G2 and DLS-G2 protocols, see Document CG-30608.
This guide provides suggestions for resolution of errors that can affect optimum performance regarding a specific sample type or workflow. These suggestions have not been validated by Bionano. Some
recommended options include the use of additional reagent volume, which may reduce the total number of reactions per kit.
This document describes the major differences between the plant DNA isolation protocols, assists users in selecting the proper DNA isolation protocol for their specific plant sample, and provides a troubleshooting guide for isolating DNA from plant tissues.
Download Plant Protocol Selection and Troubleshooting Guide
Here you will find all documentation to support our kits, consumables, and instrumentation.
View Documentation
Browse our support video library for training, tutorials, and how to demos for our products.
View Videos
View a list of documents, educational and tutorial videos to support our software products.
View List