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This SP-G2 Bone Marrow Aspirate (BMA) DNA Isolation Kit can provide ultra-high molecular weight (UHMW) genomic DNA (gDNA) in approximately five hours from 1.5 million white blood cells (WBC) of frozen human bone marrow aspirates (BMA). It utilizes an improved lyse, bind, wash, and elute procedure that is common for silica-based gDNA extraction technologies in combination with a novel paramagnetic disk. Unlike magnetic beads and silica spin columns, which shear large gDNA, the Nanobind Disk binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA.

kit contents

PART NO. Component Volume Storage SDS
20442 RBC Lysis Solution 2 x 18 ml 15-30°C Download
20374 Cell Buffer 50 ml 15-30°C Download
20457 Digestion Enhancer 6.5 ml 15-30°C Download
20458 Lysis and Binding Buffer (LBB) 2.0 ml 15-30°C Download
20459 Wash Buffer 1 (WB1) 13.0 ml 15-30°C Download
20460 Wash Buffer 2 (WB2) 17.5 ml 15-30°C Download
20463 Elution Buffer (EB) 3.6 ml 15-30°C Download
20545 DE Detergent 110 μl 15-30°C Download
20539 4mm Nanobind Disks 24 ea. 15-30°C
20540 Protein LoBind Microcentrifuge Tubes, 1.5 ml 2 x 24 ea. 15-30°C
20541 Protein LoBind Microcentrifuge Tubes, 0.5 ml 24 ea. 15-30°C
20543 Magnetic Retriever Plastic Sheath 24 ea. 15-30°C
20542 Microcentrifuge Tubes, 2 ml 24 ea. 15-30°C
20469 DNA Stabilizer 700 μl 15-30°C Download
20462 RNase A 300 μl 2°C-8°C Download
20532 Ultra Pure Water 2 x 1800 μl 2°C-8°C Download
20456 Thermolabile Proteinase K (TLPK) 325 μl -15°C to -25°C Download
2 x 20464 BMA Filter 24 ea. 15-30°C
2 x 20452 Microcentrifuge Tubes, 2 ml 2 x 12 ea. 15-30°C
2 x 20398 DNA Stabilizer 4 ml 15-30°C Download

related support materials

Bionano Prep SP-G2 Frozen Human Bone Marrow Aspirate (BMA) DNA Isolation Protocol - CG-00007​, Rev D

Generation 2 SP DNA Isolation Protocol. To be used only with PN 90151 – Bionano Prep SP-G2 Bone Marrow Aspirate DNA Isolation Kit.

Also available in French, German, Italian, and Spanish.

Bionano Prep SP-G2 Fresh Human Bone Marrow Aspirate (BMA) DNA Isolation Protocol - CG-00062, Rev A

Generation 2 SP DNA Isolation Protocol. To be used only with PN 90151 – Bionano Prep SP-G2 Bone Marrow Aspirate DNA Isolation Kit.

Frozen Bone Marrow Aspirate Collection, Storage, and Shipping Instructions - CG-30358, Rev B

Fresh Bone Marrow Aspirate Collection, Storage, and Shipping Instructions - CG-00073, Rev A

HemoCue WBC Analyzer Demo

To view this video in another language, please click on one of the available languages below:
French | German
Italian | Spanish

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