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Bionano Prep SP BMA DNA Isolation Protocol can provide ultra-high molecular weight (UHMW) gDNA from white blood cells in heparin BMA samples in as little as 4 hours. BMA samples are pre-processed by filtering to remove any bone fragments and other insoluble or non-cellular particulates. The kit utilizes a lyse, bind, wash, and elute procedure with a novel paramagnetic Nanobind disk, which binds and releases gDNA with significantly less fragmentation, resulting in UHMW gDNA. With this protocol, up to eight BMA samples can be processed in a day* with typical yields from 1.5 million cells as high as 7 ug. While fresh BMA samples can be processed with this kit, we have observed higher molecule N50s (> 400 kbp) when samples are frozen with DNA stabilizer and processed after a single freeze-thaw cycle. *Processing time may vary depending upon the number of times filtered samples need to be diluted in order to be counted accurately. Once 1.5 million cells are obtained, the total processing time is less than 4 hours.

Kit Contents

Part No. Component Volume Storage SDS
20373 RNase A Enzyme 200 μl 4°C Download
20423 / 20397 DNA Stabilizer 350 μl 18-25°C Download
20374 Cell Buffer 50 ml 18-25°C Download
20372 Proteinase K Enzyme 0.5 ml 18-25°C Download
20375 Lysis and Binding Buffer (LBB) 2.5 ml 18-25°C Download
20376 Wash Buffer 1 Concentrate (2.5X) (WB1) 3.25 ml 18-25°C Download
20377 Wash Buffer 2 Concentrate (2.5X) (WB2) 5 ml 18-25°C Download
20378 Elution Buffer (EB) 1.1 ml 18-25°C Download
20402 4 mm Nanobind Disks 10 disks 18-25°C
20422 Protein LoBind Tubes, 1.5 mL 10 tubes 18-25°C
20421 Protein LoBind Tubes, 0.5 mL 10 tubes 18-25°C
20396 Standard Microfuge Tubes, 2.0 ml 10 tubes 18-25°C
20381 Magnetic Disk Retriever Plastic Sheath 10 each 18-25°C
20398 DNA Stabilizer 4 ml 18-25°C Download
20396 Standard Microfuge Tubes, 2.0 ml 2 x 10 tubes 18-25°C
20401 BMA Filters, 100 μm 20 filters 18-25°C

related support materials

Bionano Prep SP BMA DNA Isolation Protocol v2 - 30399, Rev B

Previous generation SP DNA Isolation Protocol. To be used only with PN 90103 – SP Bone Marrow Aspirate (BMA) DNA Isolation Kit v2.
Also available in French, German, Italian, and Spanish

Bone Marrow Aspirate Collection, Storage, and Shipping Instructions - 30358, Rev A

HemoCue WBC Analyzer Demo

To view this video in another language, please click on one of the available languages below:
French | Spanish | Italian | German

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