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The Bionano Prep DLS-G2 kit provides reagents for sequence-specific labeling of UHMW gDNA for Bionano Optical Genome Mapping (OGM) on the Saphyr® system. After sequence-specific labeling with DLE-1, the labeled DNA is stained for backbone visualization. DL-Green fluorophores are seen as green labels on a blue molecule when imaged on the Saphyr® instrument.

kit contents

PART NO. Component Volume Storage SDS
20430 10x DLE-1 44 μl -25°C to -15°C Download
20429 20x DL-Green 22 μl -25°C to -15°C Download
20428 5x DLE-1 Buffer 175 μl -25°C to -15°C Download
20356 DNA Stain 65 μl -25°C to -15°C Download
20432 10x DTT 90 μl -25°C to -15°C Download
20434 Proteinase K 60 μl 2°C to 8°C Download
20431 4x Flow Buffer 225 μl 2°C to 8°C Download
20355 Ultra Pure Water 900 μl 2°C to 30°C Download
20357 DLS 24 Well Plate 1 plate 15°C to 30°C
20358 DLS Membranes 25 ea 15°C to 30°C
20433 DLS Tape Sheets 12 ea 15°C to 30°C
20437 DLS Amber Tubes 14 ea 15°C to 30°C

related support materials

Bionano Prep Direct Label and Stain-G2 (DLS-G2) Protocol - CG-30553-1, Rev D

Generation 2 DLS Labeling Protocol. To be used only with PN 80046 – DLS-G2 DNA Labeling Kit.

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Bionano Prep DLS-G2 Membrane Workflow - CG-00013, Rev A

DLS Master Mix Mixing

This video is also available in:
French | German | Italian | Spanish

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