Redefine Quality Control for Cell Bioprocessing with Optical Genome Mapping
Whether you are working with producer cell lines, research cell lines, or cell therapy applications, ensuring the genomic integrity and stability of your cell lines is of critical importance. Traditional cytogenetic methods have significant limitations in resolution, turnaround time, and scalability. Optical genome mapping (OGM) with the Bionano Saphyr® system is an advanced digital workflow that enables genome-wide analysis of structural variants (SVs) and copy number variants (CNVs), so you can easily screen cell lines for genomic instability and off-target events.
Screen your cell lines for all classes of structural variants in a single assay.
- OGM can detect aneuploidy, deletions, duplications, inversions, translocations, and repeat expansions or contractions
- Resolution 10,000x higher compared to traditional karyotyping
- Generate high-coverage data to detect low allele fractions (down to 5% with 400x coverage, currently available, and higher-coverage protocol under development)
Lower your costs and turnaround times by bringing the OGM assay in-house.
- Easy to implement in-house
- Consolidation of multiple tests drives cost savings
- Sample to answer in <1 week
Gain control over your cell QC operations.
- Fully digital workflow designed to scale
- Complete end-to-end solution including analysis software
- High-touch support from Bionano specialists
Advantages of Optical Genome Mapping Compared to Traditional Techniques
| Karyotype | Chromosomal Microarray | OGM | |
| Average TAT |
2+ weeks |
<1 week |
<1 week |
| Resolution |
5-10 Mbp |
>50-100 kbp |
>500 bp |
| Detects deletions and duplications |
(>5-10 Mbps) |
|
|
| Detects translocations and inversions |
(>5-10 Mbps) |
|
|
| Detects repeat instability | |||
| Scalable digital analysis | |||
| Easy to perform in-house |
Cell Quality Control Brochure
Learn how OGM can fit into your bioprocessing quality control workflows, while improving data quality and reducing cost and turnaround time.
DownloadHere’s How other labs have implemented OGM for Cell Line Screening and Quality Control
Redefine Quality Control for Cell Bioprocessing with Optical Genome Mapping
| Organization | Application | Methods | Findings |
|
CIRA Foundation (Japan) |
Evaluating the effects of CRISPR-Cas9 gene editing | Performed a stringent genomic integrity assessment of CRISPR-Cas9 edited IPSC subclones, using WGS, karyotyping and OGM | OGM uniquely identified unexpected chromosomal translocations and inversions introduced by gene editing |
| Oklahoma Medical Research Foundation (USA) | Evaluating the effects of prolonged cell culture on induced pluripotent stem cells (iPSCs) | Measured the effects of cell culturing in two iPSC lines in parallel for 50 passages and examined them at multiple time points using OGM | OGM identified substantial changes in the iPSC line genomes, including deletions, insertions, balanced translocations and inversions |
| Verve Therapeutics (USA)³ | Evaluating genomic integrity after (CRISPRcas) genome engineering in a primary liver cell line used in drug development | Assessed for chromosomal rearrangements and large insertions or deletions in a liver cell line treated with a single course gene editing drug in development | They showed that no additional SVs after treatment when accumulated compared with untreated controls |
| bit.bio (UK)* | Cytogenetic quality control of iPSCs | Assessed the cytogenetic health of IPSC banks at commercial scale | They adopted OGM in-house as a single workflow solution, replacing an outsourced two-assay process, reducing TAT from 5 weeks to 1 week and improving the quality of SV data |
References : 1. Kitano et al. Mol Ther Methods Clin Dev. 2022 : 26 : 15-25. doi : https://doi.org/10.1016/j.omtm.2022.05.010 2. Dubose et al. Genes. 2022; 13(7) : 1157. doi : https://www.mdpi.com/2073-4425/13/7/1157 3. Verve Therapeutics press release. April 26, 2022. https://ir.vervetx.com/news-releases/news-release-details/verve-therapeutics-presents-comprehensive-analyses-target 4. Bitbio website. May 5, 2022. https://www.bit.bio/blog/how-our-culture-enables-new-technologies
Discover How OGM Benefits Cell Bioprocessing Applications
Cytogenetic QC at bit.bio: How a next-generation cytogenetics platform enhances quality control of next-generation cells.
bit.bio
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Vigor and reproducibility in research: The cell line saga: is OGM the answer?
UT Health – San Antonio
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Sign me up!RELATED MATERIALS
| Title | Source | Authors |
|---|---|---|
| Optical Genome Mapping for Genome-Wide Structural Variation Analysis in Hematologic Malignancies: A Prospective Study Demonstrates Additional Findings Compared to Standard-of-Care (SOC) Cytogenomic Methods |
Blood January 16, 2024 |
Stephen J. Wicks, Marissa Younan, Trilochan Sahoo, et al. |
| Optical Genome Mapping Combined with High-Throughput Sequencing Is Effective for the Diagnostic and Prognostic Genomic Classification of Acute Myeloid Leukemia and Myelodysplastic Neoplasms |
Blood January 16, 2024 |
Yannick LE Bris, Romain Madeuf, Catherine Godon, et al. |
| Optical Genome Mapping Allows Detection and Characterization of Cytogenetically Cryptic Oncogenic Fusions in Pediatric Acute Myeloid Leukemia |
Blood January 16, 2024 |
Gordana Raca, Alexandra E. Kovach, Karin Miller, et al. |
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Teach Me in 10: Accelerating Cell and Gene Therapy Development – Structural Assessment of the Genome with Optical Genome Mapping
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Dr. Jeanne Loring: Unveiling the Promise of iPSCs in Therapy Research & Development with Optical Genome Mapping
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Related Focus Areas & Applications
- Kitano et al. Mol Ther Methods Clin Dev. 2022; 26:15-25. doi: https://doi.org/10.1016/j.omtm.2022.05.010
- Dubose et al. Genes. 2022; 13(7):1157. doi: https://www.mdpi.com/2073-4425/13/7/1157
- Verve Therapeutics press release. April 26, 2022. https://ir.vervetx.com/news-releases/news-release-details/verve-therapeutics-presents-comprehensive-analyses-target
- Bit.bio website. May 5, 2022. https://www.bit.bio/blog/how-our-culture-enables-new-technologies
